Evidence map›Paper›PMID 42744888›Full record

ArticleScientific reports2026

Surface dielectric barrier discharge plasma priming enhances salt-tolerant wheat germination through predicted AMY1.1 redox stabilization.

Aeshah A Awaji, Khaled Lotfy, Essam F El-Hashash, Salem Mesfir Al-Qahtani, Karima Mohamed El-Absy, Eid Abd-Elfattah Eid, Sayed A Abo-Marzoka

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Aeshah A AwajiDepartment of Biology, Faculty of Science, University of Tabuk, Tabuk, 71491, Kingdom of Saudi Arabia.
Khaled LotfyDepartment of Biology, Faculty of Science, Branch of Tayma, University of Tabuk, P.O. Box 741, Tabuk, 71491, Kingdom of Saudi Arabia.
Essam F El-HashashAgronomy Department, Faculty of Agriculture, Al-Azhar University, Cairo, 11651, Egypt. dressamelhashash5@gmail.com.
Salem Mesfir Al-QahtaniDepartment of Biology, Faculty of Science, Branch of Tayma, University of Tabuk, P.O. Box 741, Tabuk, 71491, Kingdom of Saudi Arabia.
Karima Mohamed El-AbsyDepartment of Biology, Faculty of Science, Branch of Tayma, University of Tabuk, P.O. Box 741, Tabuk, 71491, Kingdom of Saudi Arabia.
Eid Abd-Elfattah EidAgronomy Department, Faculty of Agriculture, Al-Azhar University, Cairo, 11651, Egypt.
Sayed A Abo-MarzokaCrop Physiology Dep, Agriculture Research Center, Field crops Research Institute, Kafr El Sheikh, Egypt.

Funding

University of Tabouk, Applied College, Tabuk, Saudi Arabia 0328-1440-S
6 · The paper itself

Abstract

Soil salinity severely constrains wheat (Triticum aestivum) germination, the developmental stage most dependent on redox-sensitive starch hydrolysis. Surface dielectric barrier discharge (SDBD) cold atmospheric plasma is a chemical-free seed-priming technology, yet the molecular basis of its transition from beneficial low-dose priming to inhibitory overexposure is unresolved. We combined a systematic SDBD dose-response (ten exposure durations, 0-180 s) under 4000 ppm NaCl with comprehensive agrophysiological profiling - germination, photosynthetic pigments, antioxidant enzymes, osmolytes, gas exchange, membrane stability, mineral nutrition and multivariate analysis - and with in silico molecular docking and protein-flexibility analysis of the high-pI germination α-amylase (AMY1.1) against three plasma-generated redox species (GSH, GSNO, ONOO⁻). Germination and salt tolerance peaked at 140 s (66.67% germination; salt-tolerance index 88%) within a statistically indistinguishable 120-160 s optimum, declining sharply at 180 s. We propose an integrated hormetic model in which moderate doses act through three hierarchical tiers - seed-surface remodelling, antioxidant and osmolyte priming, and predicted AMY1.1 stabilization by GSH and GSNO - whereas excessive doses shift the chemistry toward ONOO⁻-associated distress. The docking analyses generate testable molecular hypotheses rather than proof. SDBD priming at ~ 140 s is a scalable, chemical-free strategy for salt-tolerant wheat establishment.

Indexed as

alpha-AmylasesGerminationPlant ProteinsPlasma GasesSalt ToleranceTriticumMolecular Docking SimulationOxidation-ReductionSeedsSodium Chloridealpha-AmylasesPlant ProteinsPlasma GasesSodium ChlorideHormetic biostimulationNon-thermal physical elicitorOsmotic challengePost-translational redox modificationStarch reserve mobilization

Identifiers

PMID42744888
PMCPMC13578447

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.