ArticlePloS one2026
Toxic and repellent effects of hemp leaf extracts and cannabidiol against adult female yellow fever mosquitoes, Aedes aegypti.
Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hemp (Cannabis sativa) derived products have emerged as potential sources of novel biopesticides. We recently demonstrated that a hemp leaf extract was toxic to larvae of the yellow fever mosquito Aedes aegypti, and that cannabidiol (CBD) was the primary larvicidal component of the extract. Here we evaluated the toxic and repellent effects of a hemp leaf extract and CBD against adult female Ae. aegypti. Toxicity was assessed using an acute topical assay in which mosquitoes received a 500 nL application to the thorax and mortality was recorded at 24 h. Repellency was then evaluated using two complementary bioassays: (i) a no-choice membrane blood-feeding assay to quantify feeding deterrence, and (ii) a mosquito airborne repellency test (MART) to assess spatial repellency. Topical application of CBD-rich hemp leaf extract induced dose-dependent mortality in adult Ae. aegypti with a LD50 of 22.13 µg/mosquito at 24 h. When CBD isolate was tested at its equivalent doses in the extract, it caused significantly lower mortality than the hemp extract. In the membrane blood-feeding repellency assay, the hemp leaf extract repelled mosquitoes in a dose-dependent manner, whereas CBD produced weaker effects even at higher equivalent doses. In the MART assay, the hemp extract did not elicit spatial repellency, suggesting low volatility of the repellent compounds. Taken together, our results demonstrate that CBD does not fully explain the adulticidal or repellent effects of the CBD-rich hemp leaf extract against adult female Ae. aegypti suggesting that other compounds contribute to the bioactivity of hemp leaf extracts against adult female mosquitoes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.