Screening of largemouth bass-derived Bacillus subtilis and synergistic regulation of growth performance, intestinal health, and resistance against Edwardsiella piscicida infection.

Yujun Zhang, Jiahao Zhang, Yuan Liang, Weimin Huang, Wenhao Wu, Xinxin Chen, Biao Jiang, Youlu Su

Abstract read

In one paragraph

Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yujun ZhangGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Engineering Technology Research Center of Biosafety and Intelligent Control for Aquatic Animals Diseases, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510222, Guangdong, China.

Jiahao ZhangGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Engineering Technology Research Center of Biosafety and Intelligent Control for Aquatic Animals Diseases, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510222, Guangdong, China.

Yuan LiangGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Engineering Technology Research Center of Biosafety and Intelligent Control for Aquatic Animals Diseases, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510222, Guangdong, China.

Weimin HuangGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Engineering Technology Research Center of Biosafety and Intelligent Control for Aquatic Animals Diseases, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510222, Guangdong, China.

Wenhao WuGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Engineering Technology Research Center of Biosafety and Intelligent Control for Aquatic Animals Diseases, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510222, Guangdong, China.

Xinxin ChenGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Engineering Technology Research Center of Biosafety and Intelligent Control for Aquatic Animals Diseases, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510222, Guangdong, China.

Biao JiangGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Engineering Technology Research Center of Biosafety and Intelligent Control for Aquatic Animals Diseases, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510222, Guangdong, China.

Youlu SuGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangdong Engineering Technology Research Center of Biosafety and Intelligent Control for Aquatic Animals Diseases, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510222, Guangdong, China. youlusu@zhku.edu.cn.

Funding

Fourth Round of Guangdong Provincial Modern Agricultural Industry Technology System Innovation Team Construction Project 2026CXTD15Guangdong Provincial Department of Science and Technology-Rural Science and Technology commissioner Project KTP20240408

6 · The paper itself

Abstract

In this study, 49 intestinal bacterial isolates were recovered from healthy Micropterus salmoides, among which five candidate Bacillus subtilis strains were screened via multi-index in vitro evaluation. An 8-week feeding trial covering six treatment groups was conducted to assess growth performance and survival upon Edwardsiella piscicida immersion challenge. Two strains (YB-4 and YB-15) with 95% disease protection rates were selected for further intestinal histological, biochemical and qRT-PCR analyses. Dietary supplementation with YB-4 and YB-15 significantly improved feed conversion efficiency, increased intestinal villus height and muscular layer thickness, enhanced tissue antioxidant capacity, and modulated inflammatory and humoral immune gene expression toward an anti-inflammatory profile. Notably, YB-4 and YB-15 exhibited tissue-specific divergent regulation of key cytokines including TGF-β and IgM, representing a rarely reported strain-dependent immune characteristic of bass-derived B. subtilis. Collectively, YB-4 and YB-15 are promising autochthonous probiotic candidates that simultaneously promote growth, maintain intestinal homeostasis and defend against E. piscicida infection, providing targeted strain-based strategies to reduce antibiotic reliance in largemouth bass intensive aquaculture.

Indexed as

Bacillus subtilisBassEdwardsiellaEnterobacteriaceae InfectionsFish DiseasesIntestinesProbioticsAnimal FeedAnimalsAquacultureCytokinesDisease ResistanceCytokinesBacillus subtilisDisease resistanceGrowth parametersMicropterus salmoides

Identifiers

PMID42742739

DOI10.1016/j.aqrep.2023.101706

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.