ArticleMolecular microbiology2026
A Genetically Encoded Marker for Imaging of Internal Plasmodium Membranes.
Article in Molecular microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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10 authors.
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Abstract
Fluorescence microscopy is a powerful tool to analyze the subcellular architecture, and long-term live-cell imaging permits the analysis of the dynamics of intracellular structures, such as membranes. Fluorescent labeling of the plasma membrane of Plasmodium, the causative agent of malaria, via membrane-resident proteins has been described, but proteins that broadly mark internal membranes are currently elusive. Alternatively, general membrane dyes can be employed to study membrane dynamics; however, we find that the membrane dye BODIPY TR Ceramide has adverse effects on cell viability during live-cell imaging. To overcome this limitation, we present IMMP (Internal Membrane Marker for Plasmodium), a 32-amino acid long peptide derived from P. falciparum PCNA1, which targets (fluorescent) protein tags to internal membranes for detection in live or fixed samples. Importantly, IMMP enables non-invasive, long-term live-cell imaging of internal membranes without affecting P. falciparum viability and is functional in blood stages of both the human malaria parasite P. falciparum and the rodent-infecting parasite P. berghei, as well as in P. berghei mosquito stages. IMMP is also compatible with advanced techniques such as expansion microscopy. Together, our results establish IMMP as a tool for internal membrane imaging in Plasmodium.
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