Evidence map›Paper›PMID 42741354›Full record

ArticleFrontiers in microbiology2026

Toward ultracentrifugation-free processing and long-term preservation of outer membrane vesicles by culture optimization, tangential flow filtration, diafiltration, and lyophilization.

Andres Silva, Ignacio Fuentes, Jessenia C Melio, Francisco Parra, Diana Majluf, Diego Rojas, Felipe M Llancalahuen, Maria J Barros, Katina Schinnerling, Fernando Gil and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Andres Silva *Laboratorio de Genética y Patogénesis Bacteriana, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile.
Ignacio Fuentes *Laboratorio de Genética y Patogénesis Bacteriana, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile.
Jessenia C MelioLaboratorio de Genética y Patogénesis Bacteriana, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile.
Francisco ParraLaboratorio de Genética y Patogénesis Bacteriana, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile.
Diana MajlufLaboratorio de Genética y Patogénesis Bacteriana, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile.
Diego RojasLaboratorio de Genética y Patogénesis Bacteriana, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile.
Felipe M LlancalahuenTranslational Research Laboratory in Intensive Care Medicine, Departamento de Medicina Intensiva, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
Maria J BarrosLaboratorio de RNAs Bacterianos, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile.
Katina SchinnerlingLaboratorio de Inmunología Traslacional, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida e Instituto de Salud Pública, Universidad Andrés Bello, Santiago, Chile.
Fernando GilSchool of Medicine, Faculty of Medicine, Universidad de los Andes, Santiago, Chile.
Iván L CalderónLaboratorio de RNAs Bacterianos, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile.
Juan A FuentesLaboratorio de Genética y Patogénesis Bacteriana, Centro de Investigación de Resiliencia a Pandemias, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Outer membrane vesicles (OMVs) are bacterial membrane nanoparticles with applications in host-microbe interaction studies, vaccines, molecular delivery, and microbial biotechnology. However, OMV workflows often rely on ultracentrifugation, limiting accessibility and process integration. Here, we evaluated a laboratory-scale workflow for

Indexed as

bacterial vesiclesdiafiltrationflagellinlyophilizationmicrobial biotechnologyOMV enrichmentouter membrane vesicles (OMVs)Salmonella Typhi

Identifiers

PMID42741354
PMCPMC13572657

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.