ArticleFrontiers in chemistry2026
A facile approach based on test strip for on-site determination of glucose in tobacco matrices.
Article in Frontiers in chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Glucose in tobacco leaves acts as a vital chemical precursor that thermally degrades and reacts with amino acids during combustion and curing to enrich aldehydes, ketones, and nitrogen-containing heterocyclic aroma compounds, thereby shaping flavor and masking harshness. However, current analytical methods for glucose quantification are limitations of high complexity, poor portability, and low eco-friendliness. Methods: A facile approach for on-site method for glucose determination in both flue-cured and fresh tobacco leaves, based on aqueous extraction followed by test-strip reflectometric analysis, was developed. Results: Design of experiments with central composite design was employed to achieve the ideal extraction conditions, with a shaking count of 70 times and a static immersion time of 36 min. The matrix effect in the aqueous tobacco extract showed a suppression effect with relative quantitative errors below 5.0%, and the test strip's enzymatic reaction exhibited high specificity for glucose. The developed method showed a good linear relationship over the range of 2.00-90.0 mg/L (y = 0.9952× + 0.0253, Discussion: This study developed a simple, portable, and green method for the determination of glucose content in different tobacco matrices, which could provide scientific reference data for tobacco quality evaluation and physiological growth monitoring.
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