ArticleArchives of insect biochemistry and physiology2026
Delivery, Persistence, and Functional Response of dsRNA in Acyrthosiphon pisum: Progress and Remaining Barriers.
Article in Archives of insect biochemistry and physiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Aphids are major agricultural pests due to their rapid reproduction, insecticide resistance, and role as vectors of plant pathogens. RNA interference (RNAi) has emerged as a promising strategy for species-specific pest control. However, RNAi in aphids remains inconsistent, limited by dsRNA instability, inefficient uptake, and variable gene silencing. Here, we investigate key biological barriers limiting RNAi efficiency by evaluating the delivery, stability, and efficacy of double-stranded (ds)RNA in the pea aphid Acyrthosiphon pisum. Feeding bioassays using food dyes and Cy3-labeled dsRNA demonstrated ingestion and persistence of fluorescent signals within the aphid midgut for up to 120 h, confirming that the feeding system effectively delivers dsRNA to the insects. Ex vivo degradation assays revealed rapid dsRNA breakdown by aphid nucleases, with complete degradation within 2 h. Formulations improved stability: chitosan-protected dsRNA resisted aphid nuclease activity, and layered double hydroxide (LDH) nanosheets induced elevated expression of RNAi-associated genes. Functional assays targeting the vacuolar H
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