Evidence map›Paper›PMID 42738554›Full record

ArticleAnimals : an open access journal from MDPI2026

Bezafibrate and Cholic Acid-Mediated Lipid Reduction Improves the Cryotolerance of Vitrified In Vivo-Derived Porcine Embryos.

Jiehuan Xu, Mengqian He, Jun Gao, Lingwei Sun, Yuan Zhou, Caifeng Wu, Shushan Zhang, Defu Zhang, Jianjun Dai

Abstract read
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In one paragraph

Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jiehuan XuShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Mengqian HeShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Jun GaoShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.ORCID 0000-0002-2372-4445
Lingwei SunShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.ORCID 0000-0002-3438-6584
Yuan ZhouShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Caifeng WuShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Shushan ZhangShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.ORCID 0000-0002-6762-1931
Defu ZhangShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Jianjun DaiShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.

Funding

National Key R&D Program of China 2021YFD1200300National Nature Fund Youth Fund of China 32302698Shanghai Academy of Agricultural Sciences Run-Up Program ZP25172
6 · The paper itself

Abstract

backgroundHigh lipid accumulation limits the cryotolerance of porcine embryos. This study aimed to identify lipid-lowering combinations during in vivo porcine morulae developing into blastocysts and evaluates their effects on post-warming embryonic development and embryo transfer (ET) pregnancy.

methodsA stable method of in vivo embryo production was established in Meishan pigs to obtain morulae. Morulae were treated with bezafibrate and cholic acid alone, in combination, or with either compound replaced by L-carnitine or niacin, respectively. Treated embryos were vitrified-warmed, followed by determinations of lipid content, cell division and ET.

resultsThe protocol achieved 95.00% synchronization, with 22.61 embryos obtained per sow in Meishan pigs. The combination of 50 μM of bezafibrate and 50 μM of cholic acid was identified as the optimal non-toxic treatment and produced the lowest lipid droplet content (

conclusionsBezafibrate combined with cholic acid reduced embryonic lipid contents and improved cryotolerance, providing a practical strategy for porcine embryo cryopreservation.

Indexed as

bezafibratecholic acidembryo transferin vivo embryolipid dropletsMeishan pigvitrification

Identifiers

PMID42738554
PMCPMC13565250

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