Effects of Fixation and Drying on the Physicochemical Quality and Aroma Profiles of Vine Tea (

The quality and flavor of vine tea are largely determined by its processing stages, which markedly influence its physical attributes and volatile organic compounds. Elucidating the dynamic changes in physicochemical and aromatic properties throughout processing is, therefore, essential for guiding optimized processing techniques and developing high-quality vine tea products. However, the specific effects of individual processing stages on quality attributes remain poorly understood. In this study, we combined assessments of color and physical properties with untargeted metabolomics, headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS), relative odor activity value (ROAV), and gas chromatography-olfactometry (GC-O) to identify key compounds contributing to vine tea quality. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were further applied to identify characteristic metabolites associated with aroma and flavor differentiation. A total of 280 volatile organic compounds were identified, among which 14 key VOCs (ROAV ≥ 1, aroma intensity ≥ 0.5) exhibited significant dynamic variation across processing stages. Notably, compounds such as β-ionone, β-myrcene, nonanal, and hexanal displayed higher ROAVs and strong aroma intensities (AI ≥ 1.0), indicating their substantial contribution to overall aroma. Furthermore, the metabolic transformation pathways-primarily including fatty acid degradation and carotenoid cleavage-and the content changes of key aroma-active compounds were inferred across different processing stages. Based on the differential accumulation patterns of the identified volatile markers, the possible involvement of fatty acid degradation and carotenoid cleavage pathways was inferred. Drying and fixation emerged as critical steps for vine tea aroma development, while the non-enzymatic degradation of fatty acids was potentially associated with the formation of its aroma characteristics. This study provides insights that may inform future efforts toward processing optimization and quality improvement of vine tea.