ArticleAnalytical and bioanalytical chemistry2026
Development of a sequential digestion workflow for dual-platform microplastic analysis in pasteurized donor human milk using µ-FTIR and Py-GC/MS.
Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Reliable determination of microplastics in complex biological matrices remains analytically challenging because of high lipid and protein content, low particle abundance, and the risk of polymer degradation during sample preparation. This study developed and evaluated a sequential digestion and extraction workflow for isolating and characterizing microplastics in pasteurized donor human milk (PDHM). The workflow combined hexane-based lipid removal, proteinase K digestion, and controlled hydrogen peroxide treatment. Particle-count recovery was assessed using polypropylene (PP), polyethylene terephthalate (PET), and nylon 6,6 fragments measuring 0.8-1.3 mm. Recoveries ranged from 96.3% to 100%, indicating minimal loss of these fragments during digestion and filtration. The recovered fragments were analyzed by µ-FTIR. Py-GC/MS-based mass recovery was not evaluated because the dimensions of the recovered reference fragments were incompatible with the capacity of the pyrolysis sample cup, precluding their quantitative transfer and analysis. Blank-corrected microplastic abundance in PDHM ranged from 2.3 to 15.3 particles per 30 mL, equivalent to 76.7-509.9 particles L⁻
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