Evidence map›Paper›PMID 42736222›Full record

ArticlePoultry science2026

Kaempferol supplementation at 150 mg/kg improves reproductive performance of aged laying hens via modulation of the gut-liver-ovary axis.

Zhichao Xu, Yuanyuan Zhou, Shiyu Zheng, Yan Ren, Wenhai Qian, Sadequllah Ahmadi, Dikai Zhang, Rifu Xu, Ning Qin, Xue Sun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zhichao XuDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
Yuanyuan ZhouDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
Shiyu ZhengDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
Yan RenLiaoning Agricultural Vocational and Technical College, Yingkou, 115009, China.
Wenhai QianDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
Sadequllah AhmadiCollege of Agriculture, Ibaraki University, Ami, 300-0393, Japan.
Dikai ZhangDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
Rifu XuDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
Ning QinDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. Electronic address: qinninglove2008@126.com.
Xue SunDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. Electronic address: XueSun1128@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The decline of egg production in aged laying hens is primarily due to age-related declines in hepatic and ovarian functions as well as changes in reproductive hormones and reduced antioxidant capacity. Kaempferol (KAE), a flavonoid compound with potent antioxidant activity also affects ovarian functions in mammals, but its role in chickens remains poorly understood. We aimed to investigate the potential of KAE to mitigate reproductive decline in aged laying hens through modulation of the gut-liver-ovary axis. A total of 400 Hy-Line Brown aged laying hens (420 days old) were randomly assigned to four groups consistent of five replicates with 20 hens per replicate and fed diets supplemented with either 0, 75, 150, or 300 mg/kg KAE. Dietary KAE significantly improved villus height and the villus height-to-crypt depth ratio in the duodenum, jejunum, and ileum.The mRNA expression of intestinal barrier-related genes (ZO-1, claudin-1, mucin, and occludin) were increased by dietary KAE, and partially enhanced intestinal absorption and barrier function. KAE also improved hepatic morphology, reduced hepatic triglyceride (TG) and total cholesterol (TC) levels, and significantly increased the expression of lipid synthesis and transport-related genes (FAS and MTTP). Moreover, the expression of yolk precursor synthesis-related genes including VTG-II, ApoVLDL-II, VLDLR, ER-α, ER-β, and PPAR-α were upregulated by KAE. Dietary KAE was effective in elevating the level of estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and progesterone (P4), and upregulating the mRNA transcripts of ER-α, ER-β, FSHR, and LHR in ovary. Additionally, KAE was effective in enhancing the ovarian antioxidant capacity, while reducing reactive oxygen species (ROS) accumulation, and inhibiting apoptosis in prehierarchical follicles. In conclusion, 150 mg/kg KAE is the optimum dose improving intestinal integrity and hepatic metabolism, promoting follicular development and steroid hormone secretion, and ultimately enhancing egg production performance by modulating the gut-liver-ovary axis in aged laying hens.

Indexed as

Aged laying hensAntioxidationGut-liver-ovary axisKaempferolReproductive performance

Identifiers

PMID42736222
PMCPMC13594826

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.