Evidence map›Paper›PMID 42736205›Full record

ArticlePoultry science2026

Genomic diversity, antimicrobial resistance, and virulence-associated characteristics of Escherichia coli recovered from poultry hatchery-box material.

Aneta Papoušková, Martina Masaříková, Jana Palkovičová, Monika Dolejská, Alois Čížek

Abstract read
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Aneta PapouškováDepartment of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic. Electronic address: papouskovaa@vfu.cz.
Martina MasaříkováDepartment of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic.
Jana PalkovičováCentral European Institute of Technology, University of Veterinary Sciences, Brno, Czech Republic; Department of Microbiology, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic.
Monika DolejskáCentral European Institute of Technology, University of Veterinary Sciences, Brno, Czech Republic; Department of Microbiology, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic.
Alois ČížekDepartment of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic; Central European Institute of Technology, University of Veterinary Sciences, Brno, Czech Republic.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hatcheries are early points of exposure of newly hatched chicks to Escherichia coli from eggshell debris, dust, meconium, and the hatchery environment. This study used descriptive genomic surveillance to characterize population structure, antimicrobial resistance, virulence-associated genes, plasmid replicons, and genetic relatedness in a purposively selected collection of E. coli recovered from hatchery-box material in a Czech commercial poultry hatchery. From 83 samples collected between 2019 and 2023, 409 isolates were recovered. Following within-sample reduction based on antimicrobial susceptibility profiles and PCR screening for selected APEC-associated virulence genes, 91 isolates enriched for cefotaxime-medium recovery or APEC-associated gene profiles were selected for Illumina whole-genome sequencing. The sequenced collection comprised 35 sequence types across nine phylogenetic groups or clades; phylogroup B1 (59/91) and ST162 (28/91) predominated. ColV-associated markers were detected in 88 isolates, acquired resistance genes in 61, and a multidrug-resistant phenotype in 31. Seventeen isolates were resistant to ceftazidime; eight carried ESBL or plasmid-mediated AmpC determinants, while eight additional isolates had the chromosomal ampC promoter substitution C-42T as the only identified determinant associated with this phenotype. Closely related isolates, including ST131, ST162, and ST675, were recovered from different samples or parent-farm groups within restricted sampling periods. These findings document genomically diverse, resistance- and virulence-associated E. coli in hatchery-box material and identify genetically related isolates that warrant investigation through structured longitudinal sampling. Because sampling was unequal and isolates were purposively selected, the findings do not provide prevalence estimates or demonstrate source, transmission, persistence, or pathogenicity.

Indexed as

APEC-associated virulence genesDay-old chickenESBL/AmpC-producing escherichia coliHatcheryWhole-genome sequencing

Identifiers

PMID42736205
PMCPMC13594450

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.