Evidence map›Paper›PMID 42735681›Full record

ArticleCancer cell2026

Spatial analysis reveals the evolving organization of IDH-mutant glioma.

Rouven Hoefflin, Alissa C Greenwald, Noam Galili Darnell, Christopher W Mount, Yali Tiomkin, L Nicolas Gonzalez Castro, Zebin Wen, Dor Simkin, Angelica B Patterson, Inna Goliand and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Rouven HoefflinDepartment of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel; Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany. Electronic address: rouven.hoefflin@uniklinik-freiburg.de.
Alissa C GreenwaldDepartment of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel; Lunenfeld-Tanenbaum Research Institute, Sinai Health, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada. Electronic address: greenwald@lunenfeld.ca.
Noam Galili DarnellDepartment of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Christopher W MountDepartment of Pathology and Krantz Family Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Yali TiomkinDepartment of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
L Nicolas Gonzalez CastroDepartment of Pathology and Krantz Family Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA; Center for Neuro-Oncology, Dana-Farber Cancer Institute, Boston, MA, USA; Department of Neurology, Brigham and Women's Hospital, Boston, MA, USA.
Zebin WenDepartment of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Dor SimkinDepartment of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Angelica B PattersonInstitute of Immunobiology, HOCH Health Ostschweiz, Cantonal Hospital St. Gallen, St. Gallen, Switzerland.
Inna GoliandDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
Ofra GolaniDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
Liat Fellus-AlyagorDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
Marina CohenDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
Kevin JosephDepartment of Neurosurgery, Medical Center - University of Freiburg, Freiburg, Germany.
Jürgen BeckDepartment of Neurosurgery, Medical Center - University of Freiburg, Freiburg, Germany.
Vidhya M RaviDepartment of Neurosurgery, Medical Center - University of Freiburg, Freiburg, Germany; 3D-Brain Models Lab for Neurodegenerative Diseases, Medical Centre-University of Freiburg, Freiburg, Germany.
Merav KedmiDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
Hadas Keren-ShaulDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
Michael WellerDepartment of Neurology, University Hospital and University of Zurich, Zurich, Switzerland.
Yoseph AddadiDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
Marian C NeidertDepartment of Neurosurgery, Cantonal Hospital St. Gallen, St. Gallen, Switzerland; Department of Neurosurgery, University Medical Center Mainz, Mainz, Germany.
Mario L SuvàDepartment of Pathology and Krantz Family Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA. Electronic address: suva.mario@mgh.harvard.edu.
Itay TiroshDepartment of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel. Electronic address: itay.tirosh@weizmann.ac.il.

Funding

Dissecting the cellular hierarchies of malignant gliomas by single-cell functional genomicsR37CA245523 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI Mario Luca Suva · 2020 to 2026
$2.8M
Deciphering heritability, plasticity and differentiation trajectories in gliomas via single-cell multi-omicsR01CA258763 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI SUVA, MARIO LUCA · 2021 to 2025
$2.8M
Cancer Neuroscience Training ProgramT32CA272386 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI Tracy T Batchelor, MICHAEL ELDON GREENBERG · 2023 to 2026
$2.1M
NCI NIH HHS R01 CA258763NCI NIH HHS R37 CA245523NCI NIH HHS T32 CA272386
6 · The paper itself

Abstract

Adult diffuse gliomas are composed of malignant cell states interwoven with the non-malignant brain microenvironment. Here, we combine spatial transcriptomics and spatial proteomics of isocitrate dehydrogenase (IDH)-mutant gliomas to define organizational principles across histological grades. In low-grade tumors, spatial organization is shaped by underlying brain anatomy. We identify a functional white-gray matter junction that restricts cortical invasion and is associated with marked changes in tumor composition and cellular phenotypes. This junction is preferentially traversed by oligodendrocyte progenitor (OPC)-like malignant cells, suggesting a role in tumor expansion. In contrast, tumors with intermediate histological features are largely disorganized, with few recurring interactions between cancer cell states and microenvironmental cell types. In high-grade tumors, hypoxia-associated structure emerges, resembling IDH-wild-type glioblastoma. Together, these findings reveal two independent axes of spatial organization-from anatomy-driven structure in low-grade tumors to hypoxia-driven organization in high-grade tumors-and establish a framework linking tumor grade to recurrent spatial interactions.

Indexed as

Brain NeoplasmsGliomaIsocitrate DehydrogenaseMutationAnimalsHumansNeoplasm GradingProteomicsSpatial AnalysisSpatial TranscriptomicsTumor MicroenvironmentIsocitrate DehydrogenaseIDH-mutant gliomaspatial omicsspatial organizationtumor invasiontumor microenvironment

Identifiers

PMID42735681
PMCPMC13639884

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.