Evidence map›Paper›PMID 42734881›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2026

Postbiotic potential of heat-inactivated Limosilactobacillus reuteri and its cell-free supernatant in protecting intestinal health of zebrafish.

Weijuan Zhong, Huanrong He, Kaihong Hu, Kunming Huang, Biao Jiang, Chun Liu, Yanhua Huang, Youlu Su, Wei Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Weijuan ZhongInnovative Institute of Animal Healthy Breeding, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.
Huanrong HeInnovative Institute of Animal Healthy Breeding, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.
Kaihong HuInnovative Institute of Animal Healthy Breeding, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.
Kunming HuangInnovative Institute of Animal Healthy Breeding, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.
Biao JiangInnovative Institute of Animal Healthy Breeding, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.
Chun LiuInnovative Institute of Animal Healthy Breeding, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.
Yanhua HuangInnovative Institute of Animal Healthy Breeding, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.
Youlu SuInnovative Institute of Animal Healthy Breeding, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China. youlusu@zhku.edu.cn.
Wei LiInnovative Institute of Animal Healthy Breeding, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China. liwei@zhku.edu.cn.ORCID http://orcid.org/0009-0009-7670-9351

Funding

Innovative Research Group Project of the National Natural Science Foundation of China 32102809
6 · The paper itself

Abstract

Postbiotics are non-viable substances derived from probiotics, offering advantages including high stability, strong safety profiles, and broad-spectrum effects. The present study aims to investigate the impact of Limosilactobacillus reuteri postbiotics on the intestinal health of zebrafish. Zebrafish were fed basal diet supplemented with either 10⁷ CFU/g heat-inactivated L. reuteri (hLR) or corresponding cell-free L. reuteri supernatant (LRs) for 14 days. Compared to the control group (CT), villus height in the zebrafish foregut and midgut were increased in LRs group. In addition, intestinal superoxide dismutase activity was significantly enhanced in hLR group and LRs group. Gut microbiota analysis revealed that both the hLR and LRs groups exhibited increased OTUs richness, altered microbial community structure at the phylum and genus levels, and reduced abundance of potential pathogenic bacteria in the gut community structure. Moreover, transcriptome analysis indicated that the functional enrichment of differentially expressed genes in the hLR group was primarily focused on immune regulation, while that in the LRs group was mainly concentrated on cell cycle regulation. The present study found that different postbiotic components of L. reuteri exerted distinct effects on zebrafish intestinal health, providing crucial evidence for the application of L. reuteri postbiotics as an effective feed additive in aquaculture.

Indexed as

IntestinesLimosilactobacillus reuteriProbioticsZebrafishAnimal FeedAnimalsBacteriaGastrointestinal MicrobiomeHot TemperatureGut microbiotaIntestinal healthLimosilactobacillus reuteriPostbioticTranscriptomeZebrafish(Danio rerio)

Identifiers

PMID42734881
PMCPMC13574741

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.