Evidence map›Paper›PMID 42734795›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Assessing Chikungunya Virus Protease Activity and Pharmacological Inhibition Using the acc-CHIK

Muhammad Arslan Rahat, Sparsh Makhaik, Jeanne A Hardy

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Muhammad Arslan RahatDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Sparsh MakhaikDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Jeanne A HardyDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA. jhardy@umass.edu.

Funding

Biotechnology Training Program in Applied Life SciencesT32GM135096 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI Jeanne Ann Hardy, Ashish A. Kulkarni · 2020 to 2026
$3.9M
NIGMS NIH HHS T32 GM135096
6 · The paper itself

Abstract

Chikungunya virus, a mosquito-borne alphavirus, poses a significant global health threat due to its rapid spread and lack of approved antiviral therapies. The viral nonstructural protein 2 protease (nsP2p) plays a critical role in processing the viral polyprotein to produce key viral enzymes, making it an attractive target for drug development. In this study, we describe the expression and purification of recombinant CHIKV nsP2 protease and establish a robust fluorogenic assay to measure its enzymatic activity using the acc-CHIK

Indexed as

Antiviral AgentsChikungunya virusCysteine EndopeptidasesFluorescent DyesPeptidesProtease InhibitorsEscherichia coliRecombinant ProteinsSubstrate SpecificityAntiviral AgentsCysteine EndopeptidasesFluorescent DyesnsP2 proteinasePeptidesProtease InhibitorsRecombinant ProteinsActivity assayAffinity purificationAlphavirusChikungunya virus proteaseCHIKVPDrug discoveryHit assessmentPlate readerRedox PAINS identification

Identifiers

PMID42734795

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.