Characterization of the Senescence-Associated Secretory Phenotype in Primary and Induced Pluripotent Stem Cell-Derived Skin Cells via Multiplex Assays.

Stefanos A Koutsoukos, Oula Kareem, Michael Ferreyros, Igor Kogut, Ganna Bilousova

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Stefanos A Koutsoukos *Department of Dermatology, University of Colorado School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, 80045, USA.

Oula Kareem *Department of Dermatology, University of Colorado School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, 80045, USA.

Michael FerreyrosGates Institute, University of Colorado Anschutz Medical Campus, Aurora, CO, 80045, USA.

Igor KogutDepartment of Dermatology, University of Colorado School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, 80045, USA.

Ganna BilousovaDepartment of Dermatology, University of Colorado School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, 80045, USA. ganna.bilousova@cuanschutz.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The senescence-associated secretory phenotype (SASP) refers to a collection of inflammatory cytokines, chemokines, and growth factors secreted by senescence cells. Senescent cells are those that have undergone growth arrest but remain metabolically active. The study of cellular senescence and SASP has become paramount for understanding aging and age-related diseases, including cancer and Alzheimer's disease. Moreover, investigating SASP is crucial for ensuring the safe and effective use of induced pluripotent stem cell (iPSC)-derived therapeutics in regenerative medicine. The following protocol provides a reproducible method to characterize SASP in primary and iPSC-derived skin fibroblasts by multiplex immunoassays.

Indexed as

Cellular SenescenceFibroblastsInduced Pluripotent Stem CellsSenescence-Associated Secretory PhenotypeSkinCells, CulturedHumansImmunoassayCellular senescenceiPSC-derived fibroblastsiPSCs (induced pluripotent stem cells)Multiplex immunoassaysPrimary fibroblastsSenescence-associated secretory phenotype (SASP)

Identifiers

PMID42734767

DOI10.1146/annurev-pathol-121808-102144

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.