Evidence map›Paper›PMID 42734755›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

A Cell Atlas of Adult Aedes aegypti Midgut Revealed by Single Nucleus/Cell RNA Sequencing.

Yingjun Cui, Xiaotian Tang, Tse-Yu Chen, Shunlong Wang, Zhiming Yuan, Han Xia, Alexander We Franz

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yingjun CuiSection of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, New Haven, CT, 06510, USA. yingjun.cui@yale.edu.
Xiaotian TangZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310030, China.
Tse-Yu ChenDepartment of Biomedical Sciences, School of Medicine, Mercer University, Savannah, GA, 31404, USA.
Shunlong WangWuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430200, China.
Zhiming YuanWuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430200, China.
Han XiaWuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430200, China.
Alexander We FranzDepartment of Pathobiology and Integrative Biomedical Sciences, University of Missouri, Columbia, MO, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-cell RNA sequencing (scRNA-seq), which revolutionized the field of biology, is used to analyze gene expression at the individual cell level, providing insights into cellular heterogeneity and rare cell populations that are often masked by traditional bulk RNA sequencing. The technique has been applied in entomology to define cellular heterogeneity and response to pathogens in insects. In this chapter, we describe how to perform scRNA-seq of adult Aedes aegypti midgut.

Indexed as

AedesSequence Analysis, RNASingle-Cell AnalysisAnimalsCell NucleusGastrointestinal TractSingle-Cell Gene Expression AnalysisAedes aegyptiMidgutMosquitoSingle-cell RNA sequencing

Identifiers

PMID42734755

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.