Evidence map›Paper›PMID 42734746›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

A Standardized Protocol for Generating iPSC-Derived Human Microglia for Functional Genomic Assays.

Jason Ngo, Emily Lee, Marta Olah, Falak Sher

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jason NgoDepartment of Neurology, Columbia University Medical Center, New York, NY, USA.
Emily LeeSchool of General Studies, Columbia University, New York, NY, USA.
Marta OlahDepartment of Neurology, Columbia University Medical Center, New York, NY, USA.
Falak SherDepartment of Neurology, Columbia University Medical Center, New York, NY, USA. fs2644@cumc.columbia.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human induced pluripotent stem cell (iPSC)-derived microglia (iMG) provide an in vitro experimental system for studying human microglial biology, neuroinflammation, and genetic risk mechanisms associated with neurological disease. This chapter describes a standardized, scalable, and reproducible protocol for the differentiation of human iPSCs into functional microglia-like cells, with particular emphasis on applications in transcriptional and epigenomic network analysis. The protocol supports high-viability floating iMG production, compatibility with pooled CRISPR perturbation approaches, and downstream multiomic profiling, including single-cell RNA sequencing, chromatin accessibility assays, and proteomics. Detailed procedures are provided for iPSC maintenance, hematopoietic progenitor cell generation, microglial maturation, functional genomics integration, and quality control.

Indexed as

Cell Culture TechniquesGenomicsInduced Pluripotent Stem CellsMicrogliaCell DifferentiationCells, CulturedEpigenomicsHumansSingle-Cell Gene Expression AnalysisATAC-seqCRISPR screeningEpigenomicsFunctional genomicsiPSC-derived microgliaPerturb-seqSingle-cell RNA sequencingStem cell differentiationTranscriptional networks

Identifiers

PMID42734746

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.