ArticleVeterinary research communications2026
Establishment of a blocking ELISA for assessing neutralizing antibody levels against porcine epidemic diarrhea virus.
Article in Veterinary research communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
A highly specific blocking ELISA was developed for serological diagnosis of porcine epidemic diarrhea (PED) using monoclonal antibodies (mAbs) against the S1 protein of PEDV. The S1 protein was expressed in HEK293F cells and used to immunize BALB/c mice. Through hybridoma screening, a high-affinity monoclonal antibody designated 3E3 was obtained. Indirect ELISA, Western blot, and immunofluorescence assays confirmed its significant neutralizing activity, with a neutralization titer of 1:2¹⁰. A blocking ELISA was subsequently established using HRP-labeled 3E3. The assay showed no cross-reactivity with other porcine viruses, including African swine fever virus (ASFV), Porcine circovirus 2 (PCV2), Porcine deltacoronavirus (PDCoV), Porcine reproductive and respiratory syndrome virus (PRRSV), Porcine rotavirus (PoRV), and Porcine transmissible gastroenteritis virus (TGEV), and exhibited good reproducibility, with intra- and inter-batch coefficients of variation below 10%. Compared with the virus neutralization test, the blocking ELISA demonstrated higher sensitivity. Results from the two methods showed a strong positive correlation, with a positive agreement of 88.63%, a negative agreement of 88.89%, and an overall agreement of 88.8%. In conclusion, the established blocking ELISA is specific, sensitive, and reliable, suitable for serological monitoring of PEDV and evaluation of vaccine-induced immunity.
Indexed as
Identifiers
42734722What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.