Evidence map›Paper›PMID 42733247›Full record

ArticleJournal of separation science2026

Determination of Enantiomers of Deschloroketamine and Its Metabolites in Wistar Rat Brains.

Natalie Paškanová, Vendulka Hrubá, Peter Frühauf, Bronislav Jurásek, Martin Kuchař, Pavel Řezanka, Michal Kohout

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PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of separation science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Natalie PaškanováForensic Laboratory of Biologically Active Substances, Department of Chemistry of Natural Compounds, University of Chemistry and Technology Prague, Prague 6, Czech Republic.
Vendulka HrubáDepartment of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology Prague, Prague 6, Czech Republic.
Peter FrühaufDepartment of Analytical Chemistry, University of Vienna, Vienna, Austria.
Bronislav JurásekForensic Laboratory of Biologically Active Substances, Department of Chemistry of Natural Compounds, University of Chemistry and Technology Prague, Prague 6, Czech Republic.
Martin KuchařForensic Laboratory of Biologically Active Substances, Department of Chemistry of Natural Compounds, University of Chemistry and Technology Prague, Prague 6, Czech Republic.
Pavel ŘezankaDepartment of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology Prague, Prague 6, Czech Republic.
Michal KohoutDepartment of Organic Chemistry, University of Chemistry and Technology Prague, Prague 6, Czech Republic.

Funding

Ministry of Interior of the Czech Republic VK01010212
6 · The paper itself

Abstract

Deschloroketamine (DXE) is a dissociative new psychoactive substance whose enantiomers and metabolites may exhibit distinct pharmacological and toxicological profiles. Reliable analytical methods enabling the chiral determination of DXE and its metabolites in biological matrices are thus essential. In this study, we evaluated supercritical fluid chromatography coupled to tandem mass spectrometry (SFC‑MS/MS) and capillary zone electrophoresis with ultraviolet detection (CZE‑UV) for the enantioselective analysis of DXE and its major metabolites in rat brain tissue. SFC using polysaccharide‑based chiral stationary phases provided baseline separation of the hydroxylated and demethylated metabolites, but severe peak distortion of DXE enantiomers prevented their quantification. Therefore, quantitative analysis was performed by CZE employing carboxymethyl‑β‑cyclodextrin as a chiral selector and a high‑sensitivity Z‑shaped detection cell. Analysis of brain tissue samples obtained following high-dose administration revealed near equimolar concentrations of the DXE enantiomers and a higher concentration of (R)-norDXE relative to (S)-norDXE. In this particular case, the combined use of SFC‑MS/MS and CZE‑UV provided a practical workflow for the chiral profiling of DXE and its metabolites in complex biological matrices.

Indexed as

BrainAnimalsBrain ChemistryChromatography, Supercritical FluidElectrophoresis, CapillaryMaleRatsRats, WistarStereoisomerismTandem Mass Spectrometrybrain tissuecapillary electrophoresischiral separationdeschloroketamine metabolitessupercritical fluid chromatography

Identifiers

PMID42733247
PMCPMC13572844

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.