Protocol for Cryopreservation of Endothelial Monolayers: An Update.

Leah A Marquez-Curtis, Ming Han Yu, Nasim Eskandari, Janet A W Elliott

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

4 authors.

Leah A Marquez-CurtisDepartment of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada.

Ming Han YuDepartment of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada.

Nasim EskandariDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.

Janet A W ElliottDepartment of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada. janet.elliott@ualberta.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding the cryobiological responses of endothelial cells is important to ensure the successful cryopreservation of vascularized tissues and organs. The endothelium, a monolayer of endothelial cells comprising the inner lining of blood vessels and other tissues such as the cornea, is sensitive to cryoinjury. In this chapter, we update our published cryopreservation protocol, which we have since validated for several more cell types. Our optimized protocol involves culturing cells on Rinzl plastic coverslips, using a combination of a permeating cryoprotectant (5% dimethyl sulfoxide) and a non-permeating cryoprotectant (6% hydroxyethyl starch), addition of 2% chondroitin sulfate, controlled-rate slow cooling, storage in liquid nitrogen, and removal of cryoprotectants immediately after thaw. Our post-thaw assessments include membrane integrity and a functional assay immediately after thaw and after extended incubation in culture conditions.

Indexed as

CryopreservationEndothelial CellsAnimalsCell Culture TechniquesCells, CulturedChondroitin SulfatesCryoprotective AgentsDimethyl SulfoxideHumansHydroxyethyl Starch DerivativesChondroitin SulfatesCryoprotective AgentsDimethyl SulfoxideHydroxyethyl Starch DerivativesAlamarBlue reductionChondroitin sulfateCryopreservationDelayed-onset cryoinjuryDimethyl sulfoxideFluorescent microscopyGraded freezingHydroxyethyl starchMembrane integrityRinzl plastic

Identifiers

PMID42732518

DOI10.1007/978-1-0716-0783-1_30

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.