Evidence map›Paper›PMID 42732517›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Microencapsulation for Cryopreservation of Mouse Preantral Follicles.

Gang Zhao, Yue Cheng, Conghui Tian, Guangzhu Shang

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Gang ZhaoLaboratory for Cryo-Biomedical Engineering, Department of Electronic Engineering and Information Science, School of Information Science and Technology, University of Science and Technology of China, Hefei, China. zhaog@ustc.edu.cn.
Yue ChengLaboratory for Cryo-Biomedical Engineering, Department of Electronic Engineering and Information Science, School of Information Science and Technology, University of Science and Technology of China, Hefei, China.
Conghui TianLaboratory for Cryo-Biomedical Engineering, Department of Electronic Engineering and Information Science, School of Information Science and Technology, University of Science and Technology of China, Hefei, China.
Guangzhu ShangLaboratory for Cryo-Biomedical Engineering, Department of Electronic Engineering and Information Science, School of Information Science and Technology, University of Science and Technology of China, Hefei, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent advances in microencapsulation for cryopreservation have transformed cell preservation through innovative hydrogel-based strategies. By leveraging the ice-inhibiting properties of three-dimensional hydrogel networks, this approach significantly reduces reliance on toxic cryoprotectants while maintaining high cell viability. The technology has demonstrated remarkable success across diverse cell types, from human mesenchymal stem cells and erythrocytes to murine reproductive tissues. This chapter presents a comprehensive protocol using mouse follicles as a model system, detailing: (1) mechanical follicle isolation, (2) centrifugal microfluidic encapsulation in alginate hydrogels, (3) synthesis of magnetothermal nanoparticles, and (4) optimized vitrification/rewarming processes. The integrated methodology combines the protective benefits of physical encapsulation with advanced nanowarming techniques, establishing a robust platform for low-toxicity, high-efficiency cryopreservation.

Indexed as

CryopreservationOvarian FollicleAlginatesAnimalsCell SurvivalCryoprotective AgentsFemaleGlucuronic AcidHexuronic AcidsHydrogelsMiceMicrofluidicsNanoparticlesVitrificationAlginatesCryoprotective AgentsGlucuronic AcidHexuronic AcidsHydrogels3D cultureAlginate hydrogelCentrifugal microfluidicsCryopreservationIce inhibitionLow-CPA vitrificationMagnetothermal rewarmingMicroencapsulationMouse preantral folliclesNanoparticle-enhanced cryopreservation

Identifiers

PMID42732517

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.