ArticlePoultry science2026
Hempseed-based diets supplemented with carbohydrase and butyric acid for broiler chickens.
Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The aim of this study was to evaluate the impacts of hempseed-based diets supplemented with butyric acid and carbohydrase enzyme blend (xylanase + β-glucanase) on growth performance, nutrient digestibility, intestinal morphology, microbial population, and blood biochemistry of broiler chickens. A total of 480 one-day-old male broiler chicks were assigned randomly to five treatments (8 replicates each; 12 birds per pen) described as follows: a corn-soybean control (C), a 16 % hempseed (H), and hempseed + carbohydrase blend enzyme (HE), hempseed + butyric acid (HBA), and hempseed + carbohydrase + butyric acid (HEBA). Sixteen percent of hempseed as a replacement for soybean and additives was administered from days 8 to 28. Hempseed diets, particularly HE and HEBA, demonstrated higher body weight gain and lower feed conversion ratio compared with the control (p < 0.05). Birds fed HEBA diet had the lowest counts of total aerobic bacteria, E. coli, and Lactobacillus, and had significant difference compared control (p < 0.05), while showed higher calcium, and phosphorus levels in their serum (p < 0.05). Hempseed-based diets enhanced intestinal morphology, with greater villus width and villus surface area in the ileum and HBA treatment improved crypt depth and villus height/crypt depth ratio in the jejunum. Apparent ileal digestibility of dry matter, gross energy, crude protein, and ether extract increased in H and HE. Overall, replacing 16 % hempseed with carbohydrase blend and butyric acid can improve nutrient utilization, intestinal health, and immune status without adverse metabolic effects, confirming hempseed's potential as a sustainable and functional ingredient for broiler nutrition.
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