ArticleSTAR protocols2026
Protocol for fixation and staining of Drosophila melanogaster embryos optimized for DNA-PAINT nanoscopy of adherens junction proteins.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Cadherin-catenin based adherens junctions are dynamic protein complexes that mediate homophilic cell-cell adhesion, strengthen cell-cell contact, and maintain cell polarity. Here, we present a protocol to prepare Drosophila embryo samples for DNA-PAINT nanoscopy to resolve nanoscale E-cadherin clusters at junctions. We describe steps for embryo collection, fixation, immunostaining, and mounting onto glass-bottom chambered slides to prepare samples suitable for DNA-PAINT nanoscopy. DNA-PAINT microscopy provides insight into the nanoscopic clustering and arrangement of E-cadherin complexes in fixed tissue. For complete details on the use and execution of this protocol, please refer to Häring et al.
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