Evidence map›Paper›PMID 42730365›Full record

ArticleAccess microbiology2026

PCR analysis of insertion sequences leads to the generation of artefact amplicons.

Clàudia Morros-Bernaus, Ethan R Wyrsch, Steven P Djordjevic, Stineke van Houte, David Sünderhauf

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Access microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Clàudia Morros-BernausEnvironment and Sustainability Institute, University of Exeter, Biosciences, Penryn, TR10 9FE, UK.ORCID https://orcid.org/0009-0003-7472-0203
Ethan R WyrschAustralian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW 2007, Australia.
Steven P DjordjevicAustralian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW 2007, Australia.
Stineke van HouteEnvironment and Sustainability Institute, University of Exeter, Biosciences, Penryn, TR10 9FE, UK.
David SünderhaufEnvironment and Sustainability Institute, University of Exeter, Biosciences, Penryn, TR10 9FE, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Insertion sequences (ISs) are small, self-mobilizing DNA elements widespread across prokaryotic genomes, including chromosomes and plasmids. IS elements frequently co-localize with antimicrobial resistance (AMR) genes and mediate their mobilization, often as part of larger genomic structures that encompass multiple IS elements and antibiotic resistance genes. In this study, we employed Polymerase Chain Reaction (PCR) to amplify DNA sequences containing two copies of an IS

Indexed as

antimicrobial resistanceESBLGenomicsmobile genetic elementsmolecular biologytransposons

Identifiers

PMID42730365
PMCPMC13567838

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.