Complex enzyme hydrolysis of okara to enrich soluble dietary fiber and peptides and insights into the dietary fiber-protein interaction mechanism.

Jingmin Sun, Shikai Zhao, Xiaoyang Sun, Yu Chen, Tingwei Zhu, Deli Wei, Lifen Zhang

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Article in Current research in food science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Jingmin SunCollege of Food Science and Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.

Shikai ZhaoCollege of Food Science and Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.

Xiaoyang SunCollege of Food Science, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, 450046, China.

Yu ChenCollege of Food Science and Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.

Tingwei ZhuCollege of Food Science and Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.

Deli WeiLuyi Chengming Food Co., Ltd., Zhoukou, Henan, 477200, China.

Lifen ZhangCollege of Food Science and Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.

Funding

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6 · The paper itself

Abstract

The interaction between dietary fiber and protein inhibits the conversion of insoluble dietary fiber (IDF) into soluble dietary fiber (SDF) and the hydrolysis of okara protein. Viscozyme® L and pepsin were used for sequential enrichment SDF and peptides, and the interaction mechanisms between the fiber and protein were investigated by analyzing the structures of IDF, SDF, peptides, and their mixed systems. After complex enzyme hydrolysis, the SDF/IDF ratio and peptide content of okara reached 0.48 and 54.38%, respectively. Compared with hydrolysis using Viscozyme® L or pepsin alone, complex enzyme hydrolysis effectively degraded IDF and protein, disrupted hydrogen bonds in SDF-peptide, and hydrolyzed the amorphous regions of IDF. The hydrolysis of the amorphous regions of IDF and the disruption of hydrogen bonds between SDF and peptide enhanced the enrichment of SDF and peptides. Overall, this study provides a practical framework for the development and utilization of okara.

Indexed as

Dietary fiberEnzymeOkaraPeptideProtein

Identifiers

PMID42729933

DOI10.1016/j.crfs.2026.101553

PMCPMC13564138

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.