ArticleNucleic acids research2026
Zinc-dependent turnover of ZIP3 transporter mRNA by trypanosome ZNK1.
Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Like other cells, parasitic and other trypanosomatids sense Zn2+ and regulate Zn2+ transport, but the mechanisms involved remained unknown. Here, we identify a trypanosome RNA-binding protein that specifically eliminates ZIP3 transporter mRNA in Zn2+-replete conditions. We first demonstrate that Trypanosoma brucei ZIP3 mRNA abundance is subject to 3'-untranslated region (3'-UTR) and Zn2+-dependent negative control. A genome-wide RNA interference library screen, using a reporter associated with the ZIP3 3'-UTR, identifies Tb927.11.9510 as a candidate Zn2+-sensor, and we name this protein Zinc Nuclear Knuckles 1 (ZNK1) since it localizes to the nucleus and contains several Zn2+-knuckle motifs. ZNK1 is conserved among trypanosomatids, and a PIN domain suggests a ribonuclease-based mechanism. We use Cas9-editing to knockout ZNK1 and observe specific accumulation of ZIP3 transcripts, and increased intracellular Zn2+, in znk1 null cells. We validate ZNK1 as a ZIP3 3'-UTR-dependent negative regulator and identify a GU-repeat motif in the ZIP3 3'-UTR that is predictive of ZNK1-based negative control. In conclusion, ZNK1 eliminates ZIP3 transporter mRNA in a Zn2+-dependent manner. We suggest that trypanosomatid ZNK1 is a highly selective zinc finger nuclease that binds GU-repeat motifs within ZIP3 3'-UTRs and degrades Zn2+ transporter mRNA only when the tandem sensor knuckle modules are coordinated with Zn2+.
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