ArticleThe Journal of eukaryotic microbiology
The Glow Up: Optimizing Growth Conditions and Carotenoid Profiling in Euglena gracilis.
Article in The Journal of eukaryotic microbiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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3 authors.
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Abstract
Until now, only five photoactive pigments have been identified in Euglena gracilis and there has been contradictory reporting of the major carotenoid. The impact of various environmental factors on the carotenoid content of E. gracilis has not been well studied. Using an improved HPLC UV-VIS method, combined with mass spectrometry, we have now identified 12 photoactive pigments from E. gracilis extracts and confirmed diadinoxanthin as the major carotenoid produced in E. gracilis. After testing cultivation media and major carbon sources (addition of glucose-2-20 g/L, ethanol-0.2%-2.0% w/v), we found that EG: JM with 0.8% ethanol increased diadinoxanthin relative production by 361% (4.29 mg/L) when compared to standard cultivation (EG media, 24 h light, PPFD 25.9 μmol/m
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