Development of an IMSA assay targeting the hly gene for detection of Listeria monocytogenes in food samples.

Abstract read

In one paragraph

Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Junxue Qiu *Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Anhui Science and Technology University, Fengyang, 233100, China.ORCID http://orcid.org/0009-0002-5635-5137

Yanping Qi *Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Anhui Science and Technology University, Fengyang, 233100, China.ORCID http://orcid.org/0000-0003-4438-1098

Xuelong ChenAnhui Province Key Laboratory of Animal Nutritional Regulation and Health, Anhui Science and Technology University, Fengyang, 233100, China. cxlandqyp@163.com.ORCID http://orcid.org/0000-0002-9683-9200

Funding

Anhui Provincial Quality Engineering Project for the New Era of Educating People (Postgraduate Education) 2024cxcysj188Grass Feeding Livestock Resource Utilization and Health Science and Technology Innovation Team 2023AH010061Major Scientific Research Project of Anhui Provincial Department of Education 2025AHGXZK20143Veterinary Science Peak Discipline Project of Anhui Science and Technology University XK-XJGF002

6 · The paper itself

Abstract

Listeria monocytogenes remains a foodborne pathogen of major global public health concern, associated with high mortality, particularly in developing countries where food safety surveillance infrastructure is limited. The hly gene, which encodes the pore-forming toxin listeriolysin O, is a highly conserved virulence determinant across all major serotypes. In this study, we developed a closed-tube isothermal multiple self-matching-initiated amplification (IMSA) assay targeting the hly gene for the specific and visual detection of L. monocytogenes. Optimized to run at 63 °C for 45 min, the assay employs SYBR Green I preloaded in the tube cap, enabling direct visual readout under UV light without the post-amplification tube opening-a feature critical for resource-limited laboratories. The assay detected all six L. monocytogenes strains tested (serotypes 1/2a, 1/2c, 4b, and 4e) without cross-reactivity against 11 non-target foodborne pathogens. In phosphate-buffered saline (PBS), the limit of detection (LOD) was 1 × 10

Indexed as

Bacterial ToxinsFood MicrobiologyHeat-Shock ProteinsHemolysin ProteinsListeria monocytogenesMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesAnimalsBacterial ProteinsBenzothiazolesDiaminesFood ContaminationLimit of DetectionQuinolinesReal-Time Polymerase Chain ReactionSensitivity and SpecificityBacterial ProteinsBacterial ToxinsBenzothiazolesDiaminesHeat-Shock ProteinsHemolysin ProteinshlyA protein, Listeria monocytogenesQuinolinesSYBR Green IFood safetyhly geneIsothermal multiple self-matching-initiated amplificationListeria monocytogenesRapid detection

Identifiers

PMID42728459

DOI10.1007/s11274-026-05259-4

What OpenQuestion holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.