Identification of novel linear antigenic epitope of IBDV VP3 recognized by monoclonal antibodies.

Chengyu He, Zhixuan Xiong, Huiping Liang, Zheng Chen, Xiangdong Wu, Huali Xie, Huansheng Wu

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Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Chengyu HeDepartment of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, PR China.

Zhixuan XiongCollege of Life Sciences, Nanchang Normal University, Nanchang, 330032, China.

Huiping LiangDepartment of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, PR China.

Zheng ChenDepartment of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, PR China.

Xiangdong WuDepartment of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, PR China.

Huali XieDepartment of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, PR China.

Huansheng WuDepartment of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, PR China. Electronic address: huanswu@jxau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aims to prepare specific monoclonal antibodies (mAbs) of IBDV VP3. We purified the recombinant VP3 protein to immunize the BALB/c mice for subsequently cell fusion. Following three rounds of sub-cloning, we successfully obtained three hybridoma cell lines (3B, 5 G, 6A) capable of stably secreting anti-VP3 mAbs with highly specificity and sensitivity. Preliminary identification using truncated Western blotting indicated that the mAbs primarily recognized the antigenic epitope in the C-terminus of VP3. Then, we employed alanine-scanning mutagenesis to precisely identify the key amino acid residues of this linear epitope as G243, R244, G246, W248 and I249. This discovery offers a new insight on the immunological characteristics of IBDV VP3 and might lay a solid basis for the advance of novel diagnostic tools based on C-terminal epitopes.

Indexed as

Antigenic epitopeInfectious bursal disease virusMonoclonal antibodyVP3

Identifiers

PMID42727340

DOI10.1016/j.psj.2026.107599

PMCPMC13589129

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.