Evidence map›Paper›PMID 42726283›Full record

ArticleArchives of microbiology2026

Characterization of a monoclonal antibody recognizing a conserved linear neutralizing epitope of pseudorabies virus gB protein.

Yumei Chen, Jie Gao, RuiRui Liu, Yunchao Liu, Jingming Zhou, Hongliang Liu, Ying Li, Yanhua Qi, Chao Liang, Enping Liu and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yumei ChenSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Jie GaoSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
RuiRui LiuSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Yunchao LiuInstitute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, People's Republic of China.
Jingming ZhouSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Hongliang LiuSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Ying LiSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Yanhua QiSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Chao LiangSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Enping LiuSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Sixuan WuSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Aiping WangSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China. pingaw@126.com.

Funding

2025 Natural Science Foundation of of Henan Province 252300421401Major Program of the National Natural Science Foundation of China 32192454
6 · The paper itself

Abstract

Pseudorabies virus (PRV), an α-herpesvirus, infects a variety of mammals. It is mainly prevalent in domestic pigs but can also infect humans, posing significant public health risks. The glycoprotein B (gB), a crucial structural protein involved in viral entry and immune recognition, is a key target for neutralizing antibodies of PRV. This study expressed gB glycoprotein to immunise mice using eukaryotic and prokaryotic systems. The hybridoma cell line secreting anti-gB antibodies was analysed, and the neutralising epitope targeted by this monoclonal antibody was identified. Six anti-PRV monoclonal antibodies were generated via the cell fusion technique. The characterization of mAbs revealed that 4A10 exhibited an IFA titer of 1:3200 and neutralization titer of 1:2

Indexed as

Antibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralEpitopesHerpesvirus 1, SuidViral Envelope ProteinsAmino Acid SequenceAnimalsEpitope MappingEpitopes, B-LymphocyteFemaleMiceMice, Inbred BALB CPseudorabiesSwineAntibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralEpitopesEpitopes, B-LymphocyteViral Envelope ProteinsHerpesviridaeMonoclonal antibodies (mAbs)NeutralizingPseudorabies virus

Identifiers

PMID42726283

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.