ArticleBiophysics reports2026
Advances in label-free dynamic imaging of live cells using fourier ptychographic microscopy.
Article in Biophysics reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-resolution and long-term dynamic imaging are essential for visualizing the spatial distribution and interaction networks of organelles within living cells. Although traditional super-resolution fluorescence microscopy achieves impressive resolutions of 20-100 nm, it faces significant challenges, including phototoxicity, photobleaching, and limited suitability for prolonged live-cell observation. These issues have driven the development of label-free imaging technologies that aim to minimize disruption to cellular physiology while providing high-resolution, non-destructive imaging. Among label-free approaches, Quantitative Phase Imaging (QPI) has emerged as a promising alternative for live-cell research by reconstructing cellular structures based on phase changes in transmitted light. In particular, Fourier ptychographic microscopy (FPM) achieves resolutions as fine as 150 nm while maintaining a large field of view, making it highly suitable for high-resolution, label-free imaging. Since its introduction in 2013, FPM has rapidly advanced, offering computational imaging capabilities that surpass conventional resolution limits. However, current systems are constrained by slow imaging speeds due to the sequential illumination of hundreds of LEDs. Here, we review the collective advancements in FPM that have transformed its capabilities over recent years. While numerous research groups have contributed to this progress, key innovations include the development of two-dimensional super-resolution FPM techniques that overcome the diffraction limit through iterative pattern optimization. Building upon these efforts, our group has introduced three-dimensional fast high-resolution Fourier microscopy, achieving 3D dynamic imaging at sub-micron resolution through computational refocusing algorithms. Collectively, these advancements establish FPM as a groundbreaking tool for real-time, high-resolution imaging of living cells, facilitating comprehensive analysis of organelle interactions and providing valuable insights into cellular functions and disease mechanisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.