ArticleBiophysics reports2026
Optimized tissue sample preparation for cryo-electron tomography using serial lift-out.
Article in Biophysics reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Structural Polymorphism of polyG Inclusions Revealed by In Situ Cryo-Electron Tomography.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
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3 authors.
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Abstract
Cryo-electron tomography (cryo-ET) can provide invaluable insights into subcellular structures in their native environment at molecular resolution. Cryo-focused ion beam (cryo-FIB) has been widely adopted to obtain thin lamellae of cells suitable for tomographic data acquisition. However, its application to tissue samples faces significant limitations. The larger sample size extends milling time, reducing sample preparation throughput. Furthermore, the need for repeated, intricate lift-out procedures adds considerable time and complexity to the workflow. The serial lift-out technique, which can prepare multiple lamellae, increases throughput and better preserves the structural integrity of the tissue sample. Here we outline the workflow of the optimized serial lift-out method and provide a detailed protocol for its implementation, exemplified by a vitrified mouse liver tissue sample.
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