Evidence map›Paper›PMID 42723343›Full record

ArticleJournal of immunology research2026

Establishing Reference Intervals for Adult Lymphocyte Subsets: Single-Platform Volume Analysis Reveals Age- and Sex-Specific Trajectories of Immunosenescence.

Linjuan Yao, Hui Fang, Shibo He, Jianchao Wang, Xin Jin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunology research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Linjuan YaoZhejiang Chinese Medical University, Hangzhou City, Zhejiang Province, China, zcmu.edu.cn.
Hui FangHangzhou Dian Medical Laboratory Center, Hangzhou City, Zhejiang Province, China.
Shibo HeHangzhou Dian Medical Laboratory Center, Hangzhou City, Zhejiang Province, China.
Jianchao WangDepartment of Clinical Laboratory, Tongde Hospital of Zhejiang Province, Hangzhou City, Zhejiang Province, China, zjtongde.com.
Xin JinDepartment of Clinical Laboratory, Tongde Hospital of Zhejiang Province, Hangzhou City, Zhejiang Province, China, zjtongde.com.ORCID https://orcid.org/0000-0003-0046-1221

Funding

Natural Science Foundation of Zhejiang Province LY23H290003
6 · The paper itself

Abstract

backgroundCurrent clinical reference intervals for lymphocyte subsets lack age- and sex-specific stratification, limiting accurate immune assessment. This study aimed to establish such stratified reference intervals in a large cohort of healthy adults aged 20-60 years.

methodsA total of 1760 healthy adults were included. The absolute counts and percentage distributions of peripheral blood (PB) lymphocyte subsets were determined via flow cytometry (FCM) using a single-platform volume method. Differences between age groups and sexes were then compared.

resultsThe absolute counts of total T cells and CD8

conclusionThis study established refined reference intervals for adult lymphocyte subsets. The physiological ageing of the immune system constitutes a nonlinear dynamic process, and the establishment of two-dimensional reference intervals based on both sex and age is recommended.

Indexed as

AgingCD8-Positive T-LymphocytesImmunosenescenceLymphocyte SubsetsAdultAge FactorsCD4-Positive T-LymphocytesFemaleFlow CytometryHumansImmunophenotypingLymphocyte CountMaleMiddle AgedReference ValuesSex Characteristicsflow cytometry (FCM)immunosenescencelymphocyte subsetsreference interval

Identifiers

PMID42723343
PMCPMC13562915

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.