ArticleNature structural & molecular biology2026
ATP-independent unfolding of ubiquitin by Ufd1 initiates Cdc48/p97-mediated substrate processing.
Article in Nature structural & molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Capturing ATR-ATRIP in the act of activation.Nature structural & molecular biology · 2026Article
- FAF1 and FAF2 enhance unfolding by p97-UFD1-NPL4 complex enabling rational design of p97 activators.The EMBO journal · 2026Article
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Authors and funding
9 authors.
Funding
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Abstract
The Cdc48 ATPase (p97 or VCP in mammals) cooperates with its cofactors Ufd1 and Npl4 to extract polyubiquitinated proteins from membranes or multisubunit complexes, promoting their proteasomal degradation. A ubiquitin molecule in the chain is unfolded in an ATP-independent manner and initiates substrate translocation through the central pore of the ATPase. How ubiquitin is unfolded remains unclear. Here we demonstrate that the UT3 domain of Ufd1 specifically interacts with two K48-linked ubiquitins and unfolds one of the ubiquitins by binding its C-terminal β-strand into a conserved hydrophobic cleft. Simultaneous binding of UT3 to two ubiquitin molecules is required to overcome the energy barrier of ubiquitin unfolding. Subsequently, the UT3-unfolded ubiquitin is captured by Npl4 and Cdc48/p97. Experiments in vitro and in cells show that unfolding-defective mutants of Ufd1 indeed compromise Cdc48/p97 function. Our results provide a reasonable explanation of how simple protein-protein interactions cause the unfolding of the remarkably stable ubiquitin.
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Registered trials
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