ReviewNeuron2026
Voltage imaging: A practical guide.
Review in Neuron, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
2 authors.
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Abstract
Recent advances in genetically encoded voltage indicators (GEVIs), optical instrumentation, and analysis software have brought voltage imaging within reach of most neuroscience labs. Voltage imaging can resolve subthreshold dynamics and action potentials across scales, from the flickering of single ion channels to large-scale neural population dynamics in behaving animals. This primer provides a practical guide to the choices involved in setting up a voltage imaging experiment, including selecting a voltage indicator, choosing excitation wavelengths and microscopy configurations, expressing the constructs in the desired cell types, and extracting information from the data. We discuss the physical principles that govern signal-to-noise ratio, the trade-offs between one-photon and two-photon excitation, and the use of structured illumination to improve contrast in tissue. Our aim is to provide a reference for new and seasoned voltage imagers on how to design effective voltage imaging experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.