Evidence map›Paper›PMID 42721255›Full record

ArticleScience (New York, N.Y.)2026

Mechanism of membrane perforation in rotavirus cell entry.

Marilina de Sautu, Conny Leistner, Tomas Kirchhausen, Simon Jenni, Stephen C Harrison

Abstract read
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In one paragraph

Article in Science (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Marilina de SautuDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.ORCID 0000-0001-5725-160X
Conny LeistnerHarvard Cryo-EM Center for Structural Biology, Harvard Medical School, Boston, MA, USA.
Tomas KirchhausenDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA.ORCID 0000-0003-0559-893X
Simon JenniDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.ORCID 0000-0001-5722-5890
Stephen C HarrisonDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.ORCID 0000-0001-7215-9393

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell entry of nonenveloped animal viruses requires translocation of a macromolecular assembly across a cellular membrane. Double-stranded RNA viruses introduce into the target cell an inner capsid particle that does not uncoat further. Instead, it extrudes capped viral mRNA by virtue of polymerase and capping activities within it. As described here, we used cryogenic electron tomography to visualize the full course of rhesus rotavirus entry, from cell attachment and virion uptake to release of the subviral particle. The cryo-tomograms and subtomogram averaging of classified subparticles link high-resolution structures of the virion and its components with time series from live-cell fluorescence microscopy. We outline the mechanism of each step in the entry process, including the membrane perforation step that transfers a subviral particle into the cytosol.

Indexed as

Cell MembraneRotavirusRotavirus InfectionsVirus InternalizationAnimalsCapsidCapsid ProteinsCell LineChlorocebus aethiopsCryoelectron MicroscopyCytosolElectron Microscope TomographyMicroscopy, FluorescenceRNA, ViralVirionCapsid ProteinsRNA, Viral

Identifiers

PMID42721255

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.