ArticleSTAR protocols2026
Protocol for the generation, viability assessment, and downstream processing of murine precision-cut skin slices.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Precision-cut tissue slices (PCTS) are an ex vivo model of viable organ sections widely used for therapeutic screening, toxicity assessment, and infection modeling. Here, we present a protocol for the generation, viability assessment, and downstream processing of murine Precision-cut Skin Slices (PCSS). We describe steps for preparing and agarose embedding skin samples, generating PCSS, maintaining them in culture, detecting lactate dehydrogenase (LDH), and quantifying ATP. We then detail procedures for RNA/protein extraction, the quantification of PCSS homogenates, and histological and TUNEL analysis of PCSS.
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