ArticleChinese journal of integrative medicine2026
Holistic Regulation of Body-Mind Acupuncture Activates Leptin Receptor-AMPK Signaling to Restore Mitochondrial Homeostasis in Chronic Fatigue Syndrome in vitro and in vivo.
Article in Chinese journal of integrative medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo elucidate the therapeutic mechanism of holistic regulation of body and mind (HRBM) acupuncture in chronic fatigue syndrome (CFS) by focusing on leptin receptor (LepR)-AMP-activated protein kinase (AMPK) signaling to address mitochondrial dysfunction and oxidative stress.
methodsA CFS rat model was established by a 4-week treadmill-based exhaustive exercise protocol. After modeling, 50 rats were randomly allocated into 5 groups (n=10), control, CFS (model), acupuncture (Acu), Acu + Allo-aca, and Acu + Allo-aca + 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) groups. Rats in the Acu group received acupuncture at bilateral Baihui (GV 20) and Zusanli (ST 36) for 30 min daily over 4 weeks. Behavioral assessments were used to confirm model validity and treatment effects. Skeletal muscle transcriptomics were applied to identify differentially expressed genes associated with mitochondrial metabolism. LepR, AMPK, phosphorylated AMPK (p-AMPK), peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), and nuclear factor erythroid 2-related factor 2 (Nrf2) were validated by RT-qPCR and Western blot analyses. In vitro, chronic fatigue-like cells were treated with EX-527 (1 µmol/L) and AICAR (500 µmol/L). Mitochondrial membrane potential was assessed using JC-1 assay, intracellular ATP and reactive oxygen species (ROS) levels were quantified, and expressions of apoptosis-related proteins, antioxidative enzymes, and mitophagy-associated proteins were evaluated by Western blot analysis. Mitochondrial ultrastructure was observed by transmission electron microscopy. Immunofluorescence and DCFH-DA probes were used to assess the LepR/AMPK co-localization and ROS dynamics.
resultsAcupuncture at GV 20 and ST 36 significantly improved fatigue-related behaviors and exercise endurance in CFS rats. Compared with the control group, rats with CFS exhibited markedly aggravated fatigue symptoms (P<0.01), whereas acupuncture treatment significantly ameliorated these deficits (P=0.0015). Transcriptomic analysis revealed that acupuncture activated LepR-AMPK signaling pathway, accompanied by enhanced mitochondrial function, increased ATP production, and improved antioxidative capacity. Furthermore, acupuncture promoted PINK1/Parkin-mediated mitophagy and stabilized mitochondrial ultrastructure. In vitro, treatment with EX-527 and/or AICAR improved mitochondrial and metabolic measures in the chronic fatigue-like cell model.
conclusionsHRBM acupuncture exerts therapeutic effects in CFS by activating LepR-AMPK signaling, thereby restoring mitochondrial bioenergetics, enhancing antioxidant defenses, and promoting mitophagy.
Indexed as
Identifiers
42720843What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.