Evidence map›Paper›PMID 42719478›Full record

ArticleACS catalysis2026

Sequence-guided engineering of a bacterial diterpene synthase induces further cyclization.

Xiuting Wei, Wenbo Ning, Pin-Shuo Huang, Jordan Nafie, Ion Ghiviriga, Jeffrey D Rudolf

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS catalysis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xiuting WeiDepartment of Chemistry, University of Florida, Gainesville, Florida 32611-7011, United States.
Wenbo NingDepartment of Chemistry, University of Florida, Gainesville, Florida 32611-7011, United States.
Pin-Shuo HuangDepartment of Chemistry, University of Florida, Gainesville, Florida 32611-7011, United States.
Jordan NafieBioTools, Inc., 5730 Corporate Way, Suite 220, West Palm Beach, Florida 33407, United States.
Ion GhivirigaDepartment of Chemistry, University of Florida, Gainesville, Florida 32611-7011, United States.
Jeffrey D RudolfDepartment of Chemistry, University of Florida, Gainesville, Florida 32611-7011, United States.

Funding

Discovery and Biosynthesis of Bacterial TerpenoidsR35GM142574 · NIGMS · UNIVERSITY OF FLORIDA · PI Jeffrey Daniel Rudolf · 2021 to 2026
$2.4M
Acquisition of a Q Exactive HF Hybrid Quadrupole-OrbitrapS10OD021758 · OD · UNIVERSITY OF FLORIDA · PI BASSO, KARI B · 2017 to 2017
$600k
NIGMS NIH HHS R35 GM142574NIH HHS S10 OD021758
6 · The paper itself

Abstract

Enzyme engineering serves as a powerful tool in biocatalysis, enabling the development of enzymes with improved stability, activity, and specificity for a range of academic, industrial, and pharmaceutical applications. However, a limited understanding of sequence-structure-function relationships in terpene synthases, the enzymes that form the complex polycyclic hydrocarbon skeletons of terpenoid natural products, presents a major challenge in predicting and engineering the products of terpene synthases. In this study, we investigated the product profiles of two bacterial variediene synthases, OdVS from

Indexed as

cyclizationDiterpene synthasemechanismmutagenesisterpenoid

Identifiers

PMID42719478
PMCPMC13557768

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.