Evidence map›Paper›PMID 42717177›Full record

ArticleAAPS PharmSciTech2026

Spray Freeze Drying for Solid Formulations of RNA Lipid Nanoparticles.

Kinnari Santosh Arte, Rachana Sapkota, Chanakya D Patil, Yijing Huang, Eric J Munson, Sajal Patel, Nicole Payton, Rushit Lodaya, Qi Tony Zhou, Li Lily Qu

Abstract read
PubMed Publisher
In one paragraph

Article in AAPS PharmSciTech, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kinnari Santosh ArteDepartment of Industrial and Molecular Pharmaceutics, College of Pharmacy, Purdue University, West Lafayette, Indiana, 47907, USA.ORCID http://orcid.org/0009-0003-4392-4895
Rachana SapkotaDepartment of Industrial and Molecular Pharmaceutics, College of Pharmacy, Purdue University, West Lafayette, Indiana, 47907, USA.ORCID http://orcid.org/0000-0001-5612-5804
Chanakya D PatilDepartment of Industrial and Molecular Pharmaceutics, College of Pharmacy, Purdue University, West Lafayette, Indiana, 47907, USA.ORCID http://orcid.org/0009-0003-5070-8920
Yijing HuangDepartment of Industrial and Molecular Pharmaceutics, College of Pharmacy, Purdue University, West Lafayette, Indiana, 47907, USA.ORCID http://orcid.org/0009-0002-1583-5767
Eric J MunsonDepartment of Industrial and Molecular Pharmaceutics, College of Pharmacy, Purdue University, West Lafayette, Indiana, 47907, USA.ORCID http://orcid.org/0000-0001-8154-6360
Sajal PatelDosage Form Design & Development, Biopharmaceutical Development, Biopharmaceuticals R&D, AstraZeneca, Gaithersburg, Maryland, 20878, USA.
Nicole PaytonDosage Form Design & Development, Biopharmaceutical Development, Biopharmaceuticals R&D, AstraZeneca, Gaithersburg, Maryland, 20878, USA.
Rushit LodayamRNA Platform, Discovery Technologies, GSK, 200 Cambridge Park Drive, Cambridge, MA, 02420, USA.ORCID http://orcid.org/0000-0003-4107-3671
Qi Tony ZhouDepartment of Industrial and Molecular Pharmaceutics, College of Pharmacy, Purdue University, West Lafayette, Indiana, 47907, USA. tonyzhou@purdue.edu.ORCID http://orcid.org/0000-0002-1438-6990
Li Lily QuDepartment of Industrial and Molecular Pharmaceutics, College of Pharmacy, Purdue University, West Lafayette, Indiana, 47907, USA. qu135@purdue.edu.ORCID http://orcid.org/0009-0008-4543-3914

Funding

Advancing innovative therapies against pandrug-resistant Gram-negative superbugsR01AI146160 · NIAID · PURDUE UNIVERSITY · PI LI, JIAN, ZHOU, QI · 2019 to 2023
$3.2M
Aerosol delivery of combinational therapeutics targeting deadly lung infectionsR01HL167828 · NHLBI · PURDUE UNIVERSITY · PI QI ZHOU · 2024 to 2026
$1.8M
NHLBI NIH HHS R01 HL167828NIAID NIH HHS R01 AI146160
6 · The paper itself

Abstract

Spray-freeze drying (SFD) is a promising drying technique for stabilizing RNA lipid nanoparticles (RNA-LNPs) by converting them into solid-state formulations. This study examined the impact of the SFD process and the widely utilized stabilizing disaccharides, such as sucrose and trehalose, on the major properties (e.g. particle size, encapsulation efficiency) of RNA-LNPs post drying. Various process parameters, such as atomization, freezing, and drying temperature, were systematically evaluated, along with the impact of incorporating annealing post freezing step. Surface area measurements, solid-state particle size analysis, scanning electron microscopy, powder X-ray diffraction and solid-state NMR spectroscopy were employed to gain deeper insights into powder characteristics and matrix mobility. Our findings demonstrated that sucrose provided superior stabilization as compared to trehalose in our tested formulations and processes. Moreover, incorporation of the annealing process enhanced LNP stability. Results from the stability study revealed that the annealed 10% and 20% sucrose formulations maintained their stability, highlighting the potential of optimized SFD processing for producing solid formulations of RNA-LNPs.

Indexed as

LipidsNanoparticlesRNAChemistry, PharmaceuticalDrug CompoundingDrug StabilityFreeze DryingLiposomesParticle SizePowdersSpray DryingSucroseTrehaloseX-Ray DiffractionLipid NanoparticlesLipidsLiposomesPowdersRNASucroseTrehaloseLipid nanoparticlesPhysical stabilitySpray freeze dryingSucroseTrehalose

Identifiers

PMID42717177

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.