ArticleScientific reports2026
Optimized air-liquid interface cultivation enhances survival and integrity of mouse colon precision-cut tissue slices.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Mouse colon precision-cut tissue slices (cPCTS) serve as valuable models for studying physiology, toxicology, and immunology. They retain a three-dimensional structure with physiological cell composition and intact metabolic and immune functions. Additionally, cPCTS support the 3R principle by reducing animal use. This study aimed to optimize cPCTS cultivation to maintain cell integrity, minimize damage, and enhance survival of the multilayer intestinal structure. We established an air-liquid interface (ALI) cultivation system, where cPCTS rest on a semi-permeable insert membrane with medium beneath and direct air exposure above. We compared the effects of different oxygen concentrations on cPCTS survival using LDH assays, TUNEL staining, qPCR, and immunohistochemistry. cPCTS preserved the characteristic colonic architecture for up to 120 h, with optimal tissue integrity maintained for at least 96 h. Compared with 80% O
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