Evidence map›Paper›PMID 42715337›Full record

ArticleScience advances2026

The CENP-A chaperone complex spatially organizes centromeres.

Hindol Gupta, Julian Haase, Yicong Wu, Jiji Chen, Warif El Yakoubi, George Karadimov, Ana Losada, Takashi Akera, Hari Shroff, Alexander E Kelly

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Dual Roads of CENP-A for Centromere Identification.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026
    Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Hindol GuptaLaboratory of Biochemistry & Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA.ORCID 0000-0002-0499-7333
Julian HaaseLaboratory of Biochemistry & Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA.ORCID 0000-0001-5891-4241
Yicong WuAdvanced Imaging and Microscopy Resource, National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD 20892, USA.ORCID 0000-0002-8819-7527
Jiji ChenAdvanced Imaging and Microscopy Resource, National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD 20892, USA.ORCID 0000-0002-4426-3035
Warif El YakoubiCell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20894, USA.ORCID 0000-0002-2859-5910
George KaradimovLaboratory of Biochemistry & Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA.ORCID 0009-0006-4095-0141
Ana LosadaChromosome Dynamics Group, Molecular Oncology Programme, Spanish National Cancer Research Centre (CNIO), Madrid 28029, Spain.ORCID 0000-0001-5251-3383
Takashi AkeraCell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20894, USA.ORCID 0000-0002-8406-9333
Hari ShroffJanelia Research Campus, HHMI, Ashburn, VA 20147, USA.ORCID 0000-0003-3613-8215
Alexander E KellyLaboratory of Biochemistry & Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA.ORCID 0000-0002-3395-6012

Funding

Role of kinetochore proteins in chromosome segregationZIABC011491 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI KELLY, ALEXANDER · 2013 to 2025
$14.6M
Laboratory of Chromosome Dynamics and EvolutionZIAHL006249 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI AKERA, TAKASHI · 2020 to 2025
$12.1M
Intramural NIH HHS ZIA BC011491Intramural NIH HHS ZIA HL006249
6 · The paper itself

Abstract

Centromeres, defined by CENP-A-containing nucleosomes, direct kinetochore assembly for spindle attachment. In mitosis, CENP-A and the constitutive centromere-associated network (CCAN) of the inner kinetochore are arranged into bipartite subdomains within clearings of chromatin. However, less is known about their architecture during interphase. We report here an unrecognized structural role for the CENP-A chaperone machinery in establishing interphase centromere architecture. In interphase, CENP-A and the CCAN assemble conserved shell-like structures that enclose a chromatin-poor central cavity. This cavity is occupied by the interphase-specific CENP-A chaperone complex, which promotes CENP-A assembly once per cell cycle. The presence of the chaperone complex, but not its CENP-A-incorporating activity, is required to generate both the shell architecture and chromatin clearing. The CCAN scaffold CENP-C exhibits radial organization throughout the structure and is essential for its formation, primarily by recruiting the HJURP chaperone. Our findings broaden the role of the CENP-A chaperone machinery to include the structural organization of interphase vertebrate centromeres, independent of CENP-A deposition.

Indexed as

CentromereCentromere Protein AMolecular ChaperonesAnimalsChromatinChromosomal Proteins, Non-HistoneDNA-Binding ProteinsHumansInterphaseKinetochoresProtein BindingCENPA protein, humanCentromere Protein Acentromere protein CChromatinChromosomal Proteins, Non-HistoneDNA-Binding ProteinsHJURP protein, humanMolecular Chaperones

Identifiers

PMID42715337
PMCPMC13557081

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.