Evidence map›Paper›PMID 42715107›Full record

ArticleSTAR protocols2026

Protocol for arbovirus infection of Aedes aegypti by intrathoracic microinjection or artificial blood feeding.

Maria Eduarda Dos Santos Pereira de Oliveira, Evaldo Joaquim de Farias Filho, Maria Júlia Brito Couto, Ellen Caroline Nobre Santos, Lívia Baldon, Álvaro Gil Araújo Ferreira, Constância Flávia Junqueira Ayres, Marcelo Henrique Santos Paiva

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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Maria Eduarda Dos Santos Pereira de OliveiraDepartment of Entomology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, PE 50740-465, Brazil.
Evaldo Joaquim de Farias FilhoDepartment of Entomology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, PE 50740-465, Brazil.
Maria Júlia Brito CoutoDepartment of Entomology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, PE 50740-465, Brazil.
Ellen Caroline Nobre SantosMosquito Vectors, Endosymbionts and Pathogen-Vector Interaction Laboratory, René Rachou Institute, Oswaldo Cruz Foundation (Fiocruz), Belo Horizonte, Minas Gerais 30190-009, Brazil; Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Lívia BaldonMosquito Vectors, Endosymbionts and Pathogen-Vector Interaction Laboratory, René Rachou Institute, Oswaldo Cruz Foundation (Fiocruz), Belo Horizonte, Minas Gerais 30190-009, Brazil.
Álvaro Gil Araújo FerreiraMosquito Vectors, Endosymbionts and Pathogen-Vector Interaction Laboratory, René Rachou Institute, Oswaldo Cruz Foundation (Fiocruz), Belo Horizonte, Minas Gerais 30190-009, Brazil.
Constância Flávia Junqueira AyresDepartment of Entomology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, PE 50740-465, Brazil.
Marcelo Henrique Santos PaivaDepartment of Entomology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, PE 50740-465, Brazil. Electronic address: marcelo.paiva@fiocruz.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for infecting adult female Aedes aegypti with arboviruses using intrathoracic microinjection or artificial blood feeding as alternative approaches. We describe steps for rearing mosquitos, virus preparation, intrathoracic microinjection, and artificial blood feeding. We then detail procedures for saliva collection, tissue dissection (midgut and carcass), and assessment of vector competence. The protocol provides practical guidance, critical experimental considerations, and validation steps to improve reproducibility and consistency across laboratory settings.

Indexed as

Environmental sciencesGeneticsMolecular Biology

Identifiers

PMID42715107
PMCPMC13573187

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.