ArticleMikrochimica acta2026
Interfacial tripedal DNA walking coupled with oxygen-depletion/ECL-RET dual quenching for femtomolar detection of Tobacco Mosaic Virus RNA in plant extracts.
Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Sensitive quantification of Tobacco Mosaic Virus RNA (TRNA) is imperative for preventing large-scale agricultural losses. In this study, we engineered a sophisticated quenching-based electrochemiluminescence (ECL) biosensor by integrating a high-performance CdTe@ZnS nanoscaffold with a target-triggered tripedal DNA walker. To ensure a robust and stable initial luminophore density, multi-walled carbon nanotubes (MWCNTs) were utilized to anchor CdTe@ZnS core-shell quantum dots, where the ZnS shell effectively passivates surface defects and prevents cadmium leakage. Upon recognition of TRNA, a catalytic hairpin assembly (CHA) and toehold-mediated strand displacement (TMSD) cascade are initiated, driving the tripedal DNA walker to autonomously navigate the electrode surface. This movement recruits a multitude of Cu
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