Evidence map›Paper›PMID 42713714›Full record

ArticleBiology open2026

A transgenic zebrafish for spatiotemporal optogenetic activation of FGF/ERK signaling.

William K Anderson, Leanne E Iannucci, Ninet Sinaii, Julia Porcino, Katherine W Rogers

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Article in Biology open, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

William K AndersonUnit on Developmental Signaling, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.ORCID 0009-0000-0538-323X
Leanne E IannucciSection on Mechanics and Tissue Remodeling Integrating Computational and Experimental Systems, National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD 20892, USA.ORCID 0000-0001-5556-4442
Ninet SinaiiBiostatistics and Clinical Epidemiology Service, Clinical Center, NIH, Bethesda, MD 20892, USA.
Julia PorcinoBiostatistics and Clinical Epidemiology Service, Clinical Center, NIH, Bethesda, MD 20892, USA.
Katherine W RogersUnit on Developmental Signaling, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.ORCID 0000-0001-5700-2662

Funding

Decoding developmental signaling during zebrafish embryogenesisZIAHD009002 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI ROGERS, KATHERINE · 2022 to 2025
$5.1M
Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentIntramural NIH HHS ZIA HD009002Intramural Research Program ZIAHD009002-01
6 · The paper itself

Abstract

Dynamic fibroblast growth factor (FGF)/extracellular signal-regulated kinase (ERK) signaling plays key roles in development, regeneration, and disease. We recently developed a zebrafish-optimized optogenetic tool, bOpto-FGF, that enables reversible activation of FGF/ERK signaling in response to blue light (∼455 nm) by fusing a zebrafish receptor tyrosine kinase domain to a blue-light-dimerizing light oxygen voltage-sensing domain. Previously, this tool was introduced into zebrafish embryos by mRNA injection. Here, we develop Tg(ubi:bOpto-FGF), a novel transgenic zebrafish ubiquitously expressing bOpto-FGF, to streamline experimental workflows. We demonstrate robust blue-light-mediated activation of FGF/ERK signaling and FGF target gene expression in early gastrulation-stage Tg(ubi:bOpto-FGF) embryos. Light-mediated signaling activation at this stage is more spatially uniform in transgenics compared to mRNA-injected embryos, and ectopic signaling in response to continuous light exposure is activated within 3 min and maintained for at least 75 min. Furthermore, Tg(ubi:bOpto-FGF) heterozygotes are light responsive from late blastula stages through at least 24 h postfertilization. Finally, we demonstrate light-mediated spatially localized FGF/ERK signaling activation within 1 min in the head, trunk, and tail at 1 day postfertilization. This transgenic line provides a powerful and convenient new strategy for spatiotemporal manipulation of FGF/ERK signaling in the vertebrate zebrafish model.

Indexed as

Extracellular Signal-Regulated MAP KinasesFibroblast Growth FactorsMAP Kinase Signaling SystemOptogeneticsSignal TransductionZebrafishAnimalsAnimals, Genetically ModifiedBlue LightGene Expression Regulation, DevelopmentalExtracellular Signal-Regulated MAP KinasesFibroblast Growth FactorsEmbryogenesisFGF/ERKMolecular optogeneticsSignalingTransgenic zebrafish

Identifiers

PMID42713714
PMCPMC13602372

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.