Evidence map›Paper›PMID 42712515›Full record

ArticleFrontiers in microbiology2026

Development and evaluation of a multiplex PCR-based dual-platform targeted sequencing framework for precise differentiation of lumpy skin disease virus.

Yuqi Wang, Yifei Wang, Zilong Bai, Suqiu Wang, Lin Wang, Wei Zhang, Zhiqiang Gao, Jing Pu, Sile Du, Congliang Deng and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Yuqi Wang *State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Yifei Wang *State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Zilong BaiScience and Technology Research Center of China Customs, Beijing, China.
Suqiu WangScience and Technology Research Center of China Customs, Beijing, China.
Lin WangScience and Technology Research Center of China Customs, Beijing, China.
Wei ZhangScience and Technology Research Center of China Customs, Beijing, China.
Zhiqiang GaoScience and Technology Research Center of China Customs, Beijing, China.
Jing PuScience and Technology Research Center of China Customs, Beijing, China.
Sile DuScience and Technology Research Center of China Customs, Beijing, China.
Congliang DengScience and Technology Research Center of China Customs, Beijing, China.
Liwei XuScience and Technology Research Center of China Customs, Beijing, China.
Wangxue WuState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Xiangpeng ZhaoScience and Technology Research Center of China Customs, Beijing, China.
Tong RenScience and Technology Research Center of China Customs, Beijing, China.
Dan WuScience and Technology Research Center of China Customs, Beijing, China.
Jiawei ZhangScience and Technology Research Center of China Customs, Beijing, China.
Qiang GuScience and Technology Research Center of China Customs, Beijing, China.
Shaolin WangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Xiju ShiScience and Technology Research Center of China Customs, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Lumpy skin disease virus (LSDV) shares over 96% genomic identity with goatpox and sheeppox viruses, presenting severe diagnostic challenges due to cross-reactivity. Methods: To address this bottleneck, we established a targeted sequencing framework integrating multiplex PCR with short-read and long-read platforms. By sequentially screening target pathogens, identifying low-homology genes, and designing short and gradient long-fragment primer pools, we evaluated these dual-platform panels using highly homologous poxvirus samples. Results: The short-read panel stably detected target viruses at inputs as low as 5.26 ×10 Conclusion: This complementary strategy seamlessly meets both laboratory demands for high-sensitivity enrichment and frontline requirements for rapid typing, providing a reliable tool for LSDV surveillance, mutation tracking, and outbreak control.

Indexed as

amplicon sequencingCapripoxvirus differentiationlong-read sequencinglumpy skin disease virusmultiplex PCRshort-read sequencing

Identifiers

PMID42712515
PMCPMC13550171

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.