Evidence map›Paper›PMID 42710659›Full record

ArticleThe Journal of biological chemistry2026

Siglec-1-targeting single domain antibodies block or enhance pathogen binding to dendritic cells.

Hendrik J Brink, Alsya J Affandi, Shirley Man, Noah C C T Dekker, Enno A Großewichtrup, Rianne G Bouma, Negisa Seyed Toutounchi, Veronique A L Konijn, Joeke G C Stolwijk, John L van Hamme and 15 more

Abstract read
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

Hendrik J BrinkDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
Alsya J AffandiDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
Shirley ManAmsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.
Noah C C T DekkerDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
Enno A GroßewichtrupDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
Rianne G BoumaDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
Negisa Seyed ToutounchiDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
Veronique A L KonijnDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
Joeke G C StolwijkDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
John L van HammeAmsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.
Katarzyna OlesekDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
Dijmphna A M HeijnenDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
Alexander FishDivision of Biochemistry, The Netherlands Cancer Institute, Oncode Institute, Amsterdam, the Netherlands.
Angeliki MoutsiopoulouDivision of Biochemistry, The Netherlands Cancer Institute, Oncode Institute, Amsterdam, the Netherlands.
Patrick H N CelieDivision of Biochemistry, The Netherlands Cancer Institute, Oncode Institute, Amsterdam, the Netherlands.
Gillian DekkersQVQ Holding BV, Utrecht, the Netherlands.
Raimond HeukersQVQ Holding BV, Utrecht, the Netherlands.
Enrico MastrobattistaDepartment of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands.
Martine J SmitAmsterdam Institute for Molecular and Life Sciences (AIMMS), Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, Vrije Universiteit, Amsterdam, the Netherlands.
Yvette van KooykDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands.
Marit J van GilsAmsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Department of Medical Microbiology and Infection Prevention, Amsterdam University Medical Center, Amsterdam, the Netherlands.
Astrid P HeikemaLeiden Centre for Applied Bioscience, University of Leiden, Leiden, the Netherlands.
Neeltje A KootstraAmsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.
Teunis B H GeijtenbeekAmsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.
Joke M M den HaanDepartment of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, the Netherlands; Amsterdam institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands; Cancer Center Amsterdam, Amsterdam, the Netherlands. Electronic address: j.denhaan@amsterdamumc.nl.

Funding

ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
NIGMS NIH HHS R01 GM129325
6 · The paper itself

Abstract

Siglec-1 (Sialoadhesin/CD169), expressed on myeloid cells, is a receptor for sialic acids that mediates adhesion to host cells and pathogens. However, certain pathogens exploit Siglec-1 binding for further dissemination and interfering with this binding may serve as a potential therapeutic intervention. Here we identified high-affinity single domain antibodies, (also known as VHHs or Nanobodies) that allo- or orthosterically modulate ligand binding of Siglec-1. VHH clone 2C2 bound directly to the ligand binding site of Siglec-1 and effectively blocked binding of a diverse set of ligands, such as ganglioside liposomes, Campylobacter jejuni, and SARS-CoV-2, to monocyte-derived dendritic cells and ex vivo Siglec-1

Indexed as

campylobacter jejuniCD169liposomesnanobodySARS-CoV-2sialoadhesinSiglec-1single-domain antibody

Identifiers

PMID42710659
PMCPMC13676974

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.