miR-6615-3p-androgen receptor axis modulates granulosa cell fate via the MDM2-p53-BCL2 pathway in hen Prehierarchical follicles.

Dikai Zhang, Zhichao Xu, Yuan Yuan Zhou, Wenhai Qian, Sadequllah Ahmadi, Rifu Xu, Shumin Zhang, Ning Qin, Xue Sun

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Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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9 authors.

Dikai ZhangDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

Zhichao XuDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

Yuan Yuan ZhouDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

Wenhai QianDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

Sadequllah AhmadiCollege of Agriculture, Ibaraki University, Ami 300-0393, Japan.

Rifu XuDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

Shumin ZhangDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.

Ning QinDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address: ningqin@jlau.edu.cn.

Xue SunDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. Electronic address: Xuesun@jlau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs (miRNAs) play critical roles in regulating follicular development and atresia by modulating autophagy, apoptosis, and steroidogenesis in granulosa cells (GCs). However, the role of specific miRNAs in these processes remains poorly understood. In this study, we focused on miR-6615-3p to explore its function and molecular mechanism in chicken Prehierarchical follicular granulosa cells (PFGCs). The expression of miR-6615-3p in chicken PFGCs was confirmed by qRT-PCR. Dual-luciferase reporter assays identified the androgen receptor (AR) gene as a direct target of miR-6615-3p. Functional analyses revealed that miR-6615-3p suppressed AR expression, promoted apoptosis, and inhibited both autophagy and steroidogenesis in PFGCs. Together, these results suggest that miR-6615-3p downregulates AR and is associated with reduced MDM2 expression, enhanced p53 activation, and decreased BCL2 expression. This indicates that it may regulate cell fate and steroid hormone synthesis, at least in part, through the MDM2-p53-BCL2 signaling pathway. This study provides new insights into the molecular mechanisms underlying chicken follicular development and highlights miR-6615-3p as a potential molecular marker for improving reproductive performance in poultry.

Indexed as

ApoptosisARAutophagyChickenmiR-6615-3p

Identifiers

PMID42710443

DOI10.1016/j.psj.2026.107717

PMCPMC13572192

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.