Protocol for measuring endocrine-disruptive effects on transcriptional bursting using single-molecule imaging in human breast cancer cells.

Pelin Yaşar, Christopher R Day, Joseph Rodriguez

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

Update of
Protocol for measuring endocrine disruptive effects on transcriptional bursting using single-molecule imaging in human breast cancer cells.2026

5 · Who and what money

Authors and funding

3 authors.

Pelin YaşarSingle Cell Dynamics Group, Epigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA. Electronic address: pelin.yasar@nih.gov.

Christopher R DaySingle Cell Dynamics Group, Epigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA. Electronic address: christopher.day@nih.gov.

Joseph RodriguezSingle Cell Dynamics Group, Epigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA. Electronic address: joseph.rodriguez@nih.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transcriptional bursts regulate gene expression by altering burst size or burst frequency. Here, we present a protocol to analyze estrogen-responsive transcriptional bursting of the TFF1 gene in human breast cancer cell lines by integrating fixed-cell single-molecule RNA fluorescence in situ hybridization (smFISH) and live-cell single-molecule imaging. We describe cell seeding, ligand dose-response treatment, and smFISH hybridization and imaging to quantify transcription sites. We then detail live-cell imaging of a TFF1-MS2 reporter and integration of both datasets to resolve how endocrine disruptors modulate bursting dynamics. For complete details on the use and execution of this protocol, please refer to Day et al.

Indexed as

Gene ExpressionIn Situ HybridizationSingle CellSingle-molecule Assays

Identifiers

PMID42709549

DOI10.1016/j.xpro.2026.104821

PMCPMC13579415

What OpenQuestion holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.